Development of an in vitro skin model

Authors

  • Dominik Škrinjar University of Maribor, Faculty of Medicine, Institute of Biomedical Sciences, Maribor, Slovenia https://orcid.org/0000-0002-1354-7705
  • Maja Sever University of Maribor, Faculty of Medicine, Institute of Biomedical Sciences, Maribor, Slovenia
  • Tina Maver University of Maribor, Faculty of Medicine, Institute of Biomedical Sciences, Maribor, Slovenia and University of Maribor, Faculty of Medicine, Department of Pharmacology, Maribor, Slovenia https://orcid.org/0000-0002-1876-7248
  • Lidija Gradišnik University of Maribor, Faculty of Medicine, Institute of Biomedical Sciences, Maribor, Slovenia https://orcid.org/0000-0003-3492-0297
  • Tanja Zidarič University of Maribor, Faculty of Medicine, Institute of Biomedical Sciences, Maribor, Slovenia https://orcid.org/0000-0002-5466-510X

DOI:

https://doi.org/10.18690/analipazu.14.1.29-47.2024

Keywords:

in vitro skin model, rheology, 3D printing, preclinical medicine, skin diseases

Abstract

In this study, we developed bioink for 3D printing incorporating human skin cells, composed of alginate, methylcellulose, and nanofibrillar cellulose in a cell medium. The 3D bioprinter allows the creation of multilayer scaffolds that simulate the natural cellular microenvironment of the skin. Due to the need for reproducibility, we stored the bioink in a freezer and thawed it later. The rheological properties of the material after thawing significantly affect its viscosity, impacting the feasibility of 3D printing and the stability of the printed scaffolds. We found that the freezing temperature notably influences these properties, which is crucial for the further development of an in vitro skin model, promising for preclinical and clinical research.

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Author Biographies

  • Dominik Škrinjar, University of Maribor, Faculty of Medicine, Institute of Biomedical Sciences, Maribor, Slovenia

    Dominik Škrinjar

    University of Maribor, Faculty of Medicine, Institute of Biomedical Sciences, Maribor, Slovenia

    e-mail: dominik.skrinjar@student.um.si

  • Maja Sever, University of Maribor, Faculty of Medicine, Institute of Biomedical Sciences, Maribor, Slovenia

    Maja Sever

    University of Maribor, Faculty of Medicine, Institute of Biomedical Sciences, Maribor, Slovenia

    e-mail: maja.sever@student.um.si

  • Tina Maver, University of Maribor, Faculty of Medicine, Institute of Biomedical Sciences, Maribor, Slovenia and University of Maribor, Faculty of Medicine, Department of Pharmacology, Maribor, Slovenia

    Tina Maver

    University of Maribor, Faculty of Medicine, Institute of Biomedical Sciences, Maribor, Slovenia and University of Maribor, Faculty of Medicine, Department of Pharmacology, Maribor, Slovenia

    e-mail: tina.maver@um.si

  • Lidija Gradišnik, University of Maribor, Faculty of Medicine, Institute of Biomedical Sciences, Maribor, Slovenia

    Lidija Gradišnik

    University of Maribor, Faculty of Medicine, Institute of Biomedical Sciences, Maribor, Slovenia

    e-naslov: lidija.gradisnik@um.si 

  • Tanja Zidarič, University of Maribor, Faculty of Medicine, Institute of Biomedical Sciences, Maribor, Slovenia

    Tanja Zidarič

    University of Maribor, Faculty of Medicine, Institute of Biomedical Sciences, Maribor, Slovenia

    e-naslov: tanja.zidaric@um.si

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Published

28.05.2024

Issue

Vol. 14 No. 1 (2024): ANALI PAZU

Section

Prispevki

How to Cite

Škrinjar, D. ., Sever, M., Maver, T., Gradišnik, L., & Zidarič, T. (2024). Development of an in vitro skin model. Anali PAZU, 14(1), 29-47. https://doi.org/10.18690/analipazu.14.1.29-47.2024

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