Prevention of Campylobacter jejuni K49/4 adhesion to porcine small intestine cell line PSI cl1 using different plant extracts

Preprečevanje adhezije Campylobacter jejuni K49/4 na celično kulturo prašičjih črevesnih celic PSI cl1 z uporabo različnih rastlinskih ekstraktov

  • Maja Šikić Pogačar University of Maribor, Faculty of Medicine
  • Anja Klančnik University of Ljubljana, Biotechnical Faculty, Department of Food Science and Technology
  • Sonja Smole Možina University of Ljubljana, Biotechnical Faculty, Department of Food Science and Technology
  • Dušanka Mičetić Turk University of Maribor, Faculty of Medicine
DOI: https://doi.org/10.18690/actabiomed.154
Keywords: Campylobacter jejuni, adhesion, anti-adhesion therapy, cell lines, phenolic compounds, plant extracts

Abstract

Purpose: The aim of this study was to investigate the in vitro anti–adhesive properties of chemically characterized ethanolic extracts from waste Pinot noir grape (GSS) skins and seeds, olive tree leaves (OE), thyme (Thymus vulgaris) prior to (TE) and its residue after (TE–R) hydrodistillation of the essential oil, as well as Alpinia katsumadai ethanolic seed extracts (SEE) and its hydrodistillation residue (hd- SEE–R) against the pig small intestine epithelial cell line, PSI cl1.

Methods: Using PSI cl1, the anti– adhesion activities of these extracts, which normally represent "waste material" and by–products from the agro– food industry, were determined. Initially, the anti–Campylobacter jejuni and cytotoxic activities of GSS, TE, TE–R, SEE and hdSEE–R were determined to avoid any interference in the anti– adhesion assay being used.

Results: The A. katsumadai extracts showed the strongest anti–adhesive activities against C. jejuni K49/4. When using TE and TE–R, C. jejuni adhesion to PSI cl1 cells was inhibited by almost 30% over a large concentration range of extracts. GSS extracts had the lowest impact on the adhesion rate of C. jejuni to PSI cl1 cells.

Conclusion: Our findings suggest that agro–food waste material and many by–products from the agro–food industry represent sources of bioactive phytochemicals that are effective at low concentrations and could be used as therapeutic agents to prevent bacterial adhesion. This represents a step towards the application of new innovative strategies to control Campylobacter contamination and infection in the food chain. We suggest that not only plant extracts, but also waste material and agro–food industry by–products can be used as promising novel therapeutic agents with possible medical and industrial applications.

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Author Biographies

Maja Šikić Pogačar, University of Maribor, Faculty of Medicine

Asist. dr., Maribor, Slovenia. E–mail: maja_sikic@yahoo.com.au

Anja Klančnik, University of Ljubljana, Biotechnical Faculty, Department of Food Science and Technology

Ljubljana, Slovenia.

Sonja Smole Možina, University of Ljubljana, Biotechnical Faculty, Department of Food Science and Technology

Ljubljana, Slovenia.

Dušanka Mičetić Turk, University of Maribor, Faculty of Medicine

Maribor, Slovenia.

Published
2021-11-29
How to Cite
Šikić Pogačar M., Klančnik A., Smole Možina S., & Mičetić Turk D. (2021). Prevention of Campylobacter jejuni K49/4 adhesion to porcine small intestine cell line PSI cl1 using different plant extracts. Acta Medico-Biotechnica, 10(2), 48-57. https://doi.org/10.18690/actabiomed.154
Issue
Vol 10 No 2 (2017)
Section
Articles

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