Biocompatibility of supercritical carbon dioxide processed poly(lactide-co-glycolide) and poly(ε-caprolactone) assessed with primary human osteoblasts

Biokompatibilnost z metodo superkritičnega ogljikovega dioksida procesiranih poli(laktid-ko-glikolida) in poli(ε-kaprolaktona) na primarnih človeških osteoblastih

  • Barbara Dariš University of Maribor, Faculty of Medicine, Institute of Biomedical Sciences
  • Polonca Ferk University of Maribor, Faculty of Medicine, Department of Pharmacology and Experimental Toxicology
  • Elena Markočič University of Maribor, Faculty of Chemistry and Chemical Engineering, Laboratory for Separation Processes
  • Željko Knez University of Maribor, Faculty of Chemistry and Chemical Engineering, Laboratory for Separation Processes
DOI: https://doi.org/10.18690/actabiomed.131
Keywords: Biocompatibility, supercritical carbon dioxide foaming, poly(lactide-co-glycolide), poly(εcaprolactone), primary human osteoblasts

Abstract

Purpose: In the present study, poly (lactide-co-glycolide) (PLGA) and poly (ε-caprolactone) (PCL) combined with hydroxyapatite (HA) were processed with supercritical CO2 (scCO2) to obtain macroporous foams.

Methods: The in vitro biocompatibility and mineralization of the foams was assessed with primary human osteoblasts (HOB).

Results: The WST-8 (Water-soluble Tetrazolium salts) test for cell proliferation activity and alkaline phosphatase (ALP) activity assay were used to estimate HOB proliferation and differentiation respectively. The proliferation activity of HOB on foamed PLGA and PCL decreased compared to non-foamed polymer, while on PLGA and PCL foamed constructs with hydroxyapatite (HA), the proliferation activity increased compared to the foamed PLGA and PCL.

Conclusions: The ALP activity on PLGA, PCL and foamed PCL was similar to the control, being less intense on PCL foamed constructs with HA, while foamed PLGA and PLGA foamed constructs with HA showed no evidence of ALP activity.

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Author Biographies

Barbara Dariš, University of Maribor, Faculty of Medicine, Institute of Biomedical Sciences

Maribor, Slovenia.

Polonca Ferk, University of Maribor, Faculty of Medicine, Department of Pharmacology and Experimental Toxicology

Maribor, Slovenia.

Elena Markočič, University of Maribor, Faculty of Chemistry and Chemical Engineering, Laboratory for Separation Processes

Maribor, Slovenia.

Željko Knez, University of Maribor, Faculty of Chemistry and Chemical Engineering, Laboratory for Separation Processes

Prof., Maribor, Slovenia. E–pošta: zeljko.knez@um.si

Published
2021-11-28
How to Cite
Dariš B., Ferk P., Markočič E., & Knez Željko. (2021). Biocompatibility of supercritical carbon dioxide processed poly(lactide-co-glycolide) and poly(ε-caprolactone) assessed with primary human osteoblasts. Acta Medico-Biotechnica, 9(1), 42-48. https://doi.org/10.18690/actabiomed.131
Issue
Vol 9 No 1 (2016)
Section
Articles