Analiza vsebnosti skupnih fenolov v skorji različnih genotipov murv (Morus sp.)
DOI:
https://doi.org/10.18690/agricsci.23.1.2Ključne besede:
poganjki murve, kemotipske razlike, sekundarni floem, listi, bioaktivne spojinePovzetek
Murve (Morus sp.) so znane po bogatem profilu bioaktivnih spojin, zlasti fenolnih spojin, katerih vsebnost se razlikuje med rastlinskimi tkivi in genotipi. V tej študiji smo proučevali vsebnost skupnih fenolov v skorji enoletnih poganjkov različnih vrst in genotipov murv. Poleg tega članek primerja vsebnost skupnih fenolov med skorjo in listi. Študija je vključevala referenčne svilogojske kultivarje vrst M. alba in M. indica, dva lokalna slovenska genotipa bele murve ter križanca med belo in rdečo murvo (M. alba × M. rubra). Enoletni poganjki so bili vzorčeni v obdobju zimskega mirovanja, nato je bila skorja olupljena, liofilizirana in zmleta v fin prah. Vsebnost skupnih fenolov je bila določena z metodo Folin-Ciocalteu. Rezultati so pokazali značilne razlike v vsebnosti skupnih fenolov med vrstami in genotipi. Najvišja vsebnost je bila ugotovljena pri križancu M. alba × M. rubra [5,14 ± 0,01 mg ekvivalenta galne kisline (GAE) g⁻¹ suhe mase (DW)], najnižja pa pri svilogojskem kultivarju ‘Kokusou’ (4,60 ± 0,08 mg GAE g⁻¹ DW). Primerjava med skorjo in listi je pokazala, da so bile koncentracije skupnih fenolov v skorji nižje kot v listih. Rezultati kažejo, da tako genetsko ozadje kot tip rastlinskega tkiva vplivata na ravni fenolnih spojin, kar poudarja potrebo po upoštevanju teh dejavnikov v prihodnjih raziskavah bioaktivnih spojin pri murvah.
Prenosi
Literatura
1. Ainsworth, E. A., & Gillespie, K. M. (2007). Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin-Ciocalteu reagent. Nature Protocols, 2(4), 875–877. https://doi.org/10.1038/nprot.2007.102
2. Batič, F., Košmrlj-Levačič, B., Martinčič, A., Cimerman, A., Turk, B., Gogala, N., Seliškar, A., Šercelj, A., & Kosi, G. (2011). Botanični terminološki slovar. ZRC SAZU.
3. Cappellozza, L. (2002). Mulberry germplasm resources in Italy. Animal Production Health Paper, 147, 97–101.
4. Chang, L. W., Juang, L. J., Wang, B. S., Wang, M. Y., Tai, H. M., Hung, W. J., Chen, Y. J., & Huang, M. H. (2011). Antioxidant and antityrosinase activity of mulberry (Morus alba L.) twigs and root bark. Food and Chemical Toxicology, 49(4), 785–790. https://doi.org/10.1016/j.fct.2010.11.045
5. Dadhwal, R., & Banerjee, R. (2023). Ethnopharmacology, pharmacotherapeutics, biomedicinal and toxicological profile of Morus alba L.: A comprehensive review. South African Journal of Botany, 158, 98-117. https://doi.org/10.1016/j.sajb.2023.05.006
6. Dai, J., & Mumper, R. J. (2010). Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules, 15(10), 7313–7352. https://doi.org/10.3390/molecules15107313
7. Fatima, M., Dar, M. A., Dhanavade, M. J., Abbas, S. Z., Bukhari, M. N., Arsalan, A., Liao, Y., Wan, J., Bukhari J. S. S., & Ouyang, Z. (2024). Biosynthesis and pharmacological activities of the bioactive compounds of white mulberry (Morus alba): Current paradigms and future challenges. Biology, 13(7), 506. https://doi.org/10.3390/biology13070506
8. IBM SPSS Statistics. (2024). IBM SPSS Statistics software (Version 29) [Computer software]. https://www.ibm.com/products/spss-statistics
9. James, A., Wang, K., Chen, Y., & Wang, Y. (2024). Functional benefits of mulberry leaf tea or extracts to alleviate metabolic diseases: Current opinion and perspectives. Food Bioscience, 59, 104218. https://doi.org/10.1016/j.fbio.2024.104218
10. Jelen, Š. (2022). Analiza fenolov v listih murv (Morus sp.) in njihov protibakterijski učinek [Master’s thesis, University of Maribor, Faculty of Agriculture and Life Sciences]. DKUM. https://dk.um.si/IzpisGradiva.php?lang=slv&id=83418
11. Jelen, Š., Kozmos, M., Senekovič, J., Ivajnšič, D., Cappellozza, S., & Urbanek Krajnc, A. (2025). Local climate and cultivation practice shape total protein and phenolic content of mulberry (Morus sp.) leaves in Sub-Mediterranean and Sub-Pannonian regions of Slovenia. Horticulturae, 11(9), 1096. https://doi.org/10.3390/horticulturae11091096
12. Kew – Board of Trustees of the Royal Botanic Gardens. (2025). Plants of the World Online. https://powo.science.kew.org/
13. Khan, M. A., Rahman, A. A., Islam, S., Khandokhar, P., Parvin, S., Islam, M. B., Hossain, M., Rashid, M., Sadik, G., Nasrin, S., Mollah, M. N. H., & Alam, A. H. (2013). A comparative study on the antioxidant activity of methanolic extracts from different parts of Morus alba L. (Moraceae). BMC Research Notes, 6, 1–9. https://doi.org/10.1186/1756-0500-6-24
14. Kimura, T., Nakagawa, K., Kubota, H., Kojima, Y., Goto, Y., Yamagishi, K., Oita, S., Oikawa, S., & Miyazawa, T. (2007). Food-grade mulberry powder enriched with 1-deoxynojirimycin suppresses the elevation of postprandial blood glucose in humans. Journal of Agricultural and Food Chemistry, 55(14), 5869-5874. https://doi.org/10.1021/jf062680g
15. Kuljarusnont, S., Iwakami, S., Iwashina, T., & Tungmunnithum, D. (2024). Flavonoids and other phenolic compounds for physiological roles, plant species delimitation, and medical benefits: A promising view. Molecules, 29(22), 5351. https://doi.org/10.3390/molecules29225351
16. Kumar, K., Debnath, P., Singh, S., & Kumar, N. (2023). An overview of plant phenolics and their involvement in abiotic stress tolerance. Stresses, 3(3), 570-585. https://doi.org/10.3390/stresses3030040
17. Li, W., Chen, L., Li, M., Peng, K., Lin, X., Feng, Y., Zou, Y., & Wu, X. (2025). Study on chemical composition, anti-inflammatory activity and quality control of the branch bark of Morus alba L. Fitoterapia, 181, 106383. https://doi.org/10.1016/j.fitote.2025.106383
18. Liu, C. H., Liu, F., & Xiong, L. (2023). Medicinal parts of mulberry (leaf, twig, root bark, and fruit) and compounds thereof are excellent traditional Chinese medicines and foods for diabetes mellitus. Journal of Functional Foods, 106, 105619. https://doi.org/10.1016/j.jff.2023.105619
19. Machii, H., Koyama, A., & Yamanouchi, H. (2002). Mulberry breeding, cultivation and utilization in Japan. Animal Production and Health Paper, 147, 63–71.
20. Memete, A. R., Timar, A. V., Vuscan, A. N., Miere Groza, F., Venter, A. C., & Vicas, S. I. (2022). Phytochemical composition of different botanical parts of Morus species, health benefits and application in food industry. Plants, 11(2), 152. https://doi.org/10.3390/plants11020152
21. Nurzyńska-Wierdak, R. (2023). Phenolic compounds from new natural sources – Plant genotype and ontogenetic variation. Molecules, 28(4), 1731. https://doi.org/10.3390/molecules28041731
22. Panek-Krzyśko, A., & Stompor-Gorący, M. (2021). The pro-health benefits of morusin administration – An update review. Nutrients, 13(9), 3043. https://doi.org/10.3390/nu13093043
23. Plants of the World Online. (2026). Royal Botanic Gardens, Kew. Available at: https://powo.science.kew.org/taxon/urn:lsid:ipni.org:names:30004492-2 (accessed 11 April 2026).
24. Saini, N., Anmol, A., Kumar, S., Wani, A. W., Bakshi, M., & Dhiman, Z. (2024). Exploring phenolic compounds as natural stress alleviators in plants-a comprehensive review. Physiological and Molecular Plant Pathology, 133, 102383. https://doi.org/10.1016/j.pmpp.2024.102383
25. Sušnik, P., Urbanek Krajnc, A., & Berčič, R. L. (2019). Murve na Slovenskem – dediščina nekdanjega svilogojstva. Proteus, 81, 306-215. http://www.dlib.si/details/URN:NBN:SI:doc-QVBZ0SBV
26. Thabti, I., Elfalleh, W., Tlili, N., Ziadi, M., Campos, M. G., & Ferchichi, A. (2014). Phenols, flavonoids, and antioxidant and antibacterial activity of leaves and stem bark of Morus species. International Journal of Food Properties, 17(4), 842-854. https://doi.org/10.1080/10942912.2012.660722
27. Wang, Y., Ai, Q., Gu, M., Guan, H., Yang, W., Zhang, M., Mao, J., Lin, Z., Liu, Q., & Liu, J. (2024). Comprehensive overview of different medicinal parts from Morus alba L.: Chemical compositions and pharmacological activities. Frontiers in Pharmacology, 15, 1364948. https://doi.org/10.3389/fphar.2024.1364948
28. WFO (2026). Morus alba L. World Flora Online. Available at http://www.worldfloraonline.org/taxon/wfo-0000447965
29. Wu, C., Wang, H., Liu, Z., Xu, B., Li, Z., Song, P., & Chao, Z. (2022). Untargeted metabolomics coupled with chemometrics for leaves and stem barks of Dioecious Morus alba L. Metabolites, 12(2), 106. https://doi.org/10.3390/metabo12020106
30. Xu, L., & Wang, X. (2025). A comprehensive review of phenolic compounds in horticultural plants. International Journal of Molecular Sciences, 26(12), 5767. https://doi.org/10.3390/ijms26125767
31. Yan, Z., Alimujiang, S., Zhang, Y., Zhao, J., Hu, Y., & Li, W. (2025). Recent advances on the chemical composition, pharmacological properties, and product development of Morus alba. Phytochemical Analysis, 36(5), 1301-1332. https://doi.org/10.1002/pca.3544
32. Zafar, M. S., Muhammad, F., Javed, I., Akhtar, M., Khaliq, T., Aslam, B., Waheed, A., Yasmin, R., & Zafar, H. (2013). White mulberry (Morus alba): A brief phytochemical and pharmacological evaluations account. International Journal of Agriculture and Biology, 15(3), 612–620. http://www.fspublishers.org
33. Zou, Y., Liao, S., Shen, W., Liu, F., Tang, C., Chen, C. Y., & Sun, Y. (2012). Phenolics and antioxidant activity of mulberry leaves depend on cultivar and harvest month in Southern China. International Journal of Molecular Sciences, 13(12), 16544–16553. https://doi.org/10.3390/ijms131216544
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Avtorske pravice (c) 2026 Evelyn Vuk, Špela jelen, Andreja Uranjek Krajnc
To delo je licencirano pod Creative Commons Priznanje avtorstva-Nekomercialno-Brez predelav 4.0 mednarodno licenco.